At　room　temperature　(21-25),　the　long　start-up　time　of　anaerobic　ammonium　oxidation　(ANAMMOX)　granular　sludge　is　the　main　problem　preventing　the　practical　application　of　this　technology.　This　experiment　aimed　to　cultivate　the　anaerobic　ammonia　oxidizing　granular　sludge　in　the　SBR　reactor　by　adjusting　the　temperature.　After　the　start-up　of　the　ANAMMOX,　stable　operation　was　achieved　by　changing　hydraulic　retention　time　(HRT)　and　the　concentration　of　the　inflow　water　substrate.　The　results　demonstrated　that　the　anaerobic　ammonia　oxidation　granular　sludge　could　be　successfully　started-up　in　30　d　and　realized　a　stable　operation　at　room　temperature　(21-25).　The　removal　rate　of　ammonia-nitrogen　and　nitrogen　was　about　88%　and　85%,　respectively,　and　the　removal　rate　of　total　nitrogen　was　75%.　At　the　stable　operation　stage　of　the　low　matrix,　the　removal　rate　of　ammonia-nitrogen　reached　86%,　the　removal　rate　of　nitrogen　was　98%,　and　the　total　nitrogen　removal　rate　was　over　85%.　SVI　was　stable　at　30　mL•g-1,　and　MLVSS/MLSS　was　more　than　60%.　The　value　of　protein/polysaccharide　was　stable　around　1.　75.　The　dissolved　oxygen　in　the　influent　was　not　removed.　In　this　case,　controlling　the　temperature　could　be　used　to　realize　the　rapid　start-up　of　the　anaerobic　ammonia　oxidation　granular　sludge　at　room　temperature　(21-25),　and　the　high　biomass　content　and　nitrogen　removal　rate　can　be　obtained.　©　2019,　Science　Press.　All　right　reserved.