The　Ni-based　superalloy　is　used　as　the　turbine　blade,　which　is　subject　to　the　coupling　effect　of　temperature　and　super-gravity　during　service.　As　the　Ni-based　superalloys　are　difficult　to　become　homogenous　after　using　the　solid　solution　heat　treatment,　a　study　on　morphology　and　composition　distribution　of　Ni-base　superalloys　with　segregation　during　microstructural　degradation　is　necessary.　This　study　investigates　the　microstructure　of　the　ex-service　turbine　blade　and　cast　samples　subjected　to　the　high-temperature　centrifugal　test.　The　difference　in　the　size　and　shape　factor　of　the　gamma　＇　phase　decreased　with　the　stress　caused　by　the　super-gravity　condition,　indicating　a　higher　magnitude　of　homogenization　degree.　The　higher　stress　will　also　promote　the　merge　of　the　sub-grain　boundaries,　leading　to　a　lower　density　and　higher　orientational　deviation　of　the　sub-grain　boundaries.