Mine　wastewater　treatment　using　bio-sulfate　reduction　technology　forms　sulfur-containing　wastewater　that　comprises　sulfides　(HS−　and　S2−)　and　metal　ions.　Bio‑sulfur　generated　by　sulfur-oxidizing　bacteria　in　such　wastewater　is　usually　negatively　charged　hydrocolloidal　particles.　However,　bio‑sulfur　and　metal　resource　recovery　are　difficult　using　traditional　methods.　In　this　study,　the　sulfide　biological　oxidation-alkali　flocculation　(SBO-AF)　method　was　investigated　to　recover　the　above　resources,　and　to　provide　a　technical　reference　for　mine　wastewater　resource　recovery　and　heavy　metal　pollution　control.　Specifically,　the　performance　of　SBO　in　forming　bio‑sulfur　and　the　key　parameters　of　SBO-AF　were　explored　and　then　applied　in　a　pilot-scale　process　to　recover　resources　from　wastewater.　Results　show　that　partial　sulfide　oxidation　was　achieved　under　a　sulfide　loading　rate　of　5.08　±　0.39　kg/m3·d,　dissolved　oxygen　of　2.9–3.5　mg/L　and　temperature　of　27–30　°C.　The　average　sulfide　oxidation　rate　and　sulfur　selectivity　ratio　were　92.86　%　and　90.22　%,　respectively.　At　pH　10,　metal　hydroxide　and　bio‑sulfur　colloids　co-precipitated　through　the　precipitation　catching　and　adsorption　charge　neutralization　effect.　The　average　manganese,　magnesium　and　aluminum　concentrations　and　turbidity　in　the　wastewater　were　53.93　mg/L,　522.97　mg/L,　34.20　mg/L　and　505　NTU,　respectively,　and　decreased　to　0.49　mg/L,　80.65　mg/L,　1.00　mg/L　and　23.33　NTU,　respectively,　after　treatment.　The　recovered　precipitate　mainly　contained　sulfur,　along　with　metal　hydroxides.　The　average　sulfur,　manganese,　magnesium　and　aluminum　contents　were　45.6　%,　29.5　%,　15.1　%　and　6.5　%,　respectively.　Economic　feasibility　analysis　and　the　above　results　show　that　SBO-AF　has　obvious　technical　and　economic　advantages　in　the　recovery　resources　from　mine　wastewater.　©　2023　Elsevier　B.V.