Intelligent　additive　manufacturing　is　the　future　direction,　and　changing　parameters　during　manufacturing　process　is　an　effective　measure　to　adjust　the　quality　of　parts.　In　this　study,　the　stability　behavior　of　the　manufacturing　process　under　three　process　strategies,　low　layer　thickness　fabrication　(LLTF),　high　layer　thickness　fabrication　based　on　the　interval　of　the　powder　layer　thickness　(IPLT),　and　changing　of　layer　thickness　strategy　(CLTS),　was　comparatively　investigated.　Multilayer　experiments　and　mechanical　properties　experiments　were　performed　during　laser　powder　bed　fusion,　and　the　correlation　between　manufacturing　quality　and　powder　layer　thickness　was　compared.　By　optimizing　the　process　parameters,　the　surface　quality　of　CLTS　is　similar　to　that　of　LLTF,　and　the　value　of　minimum　surface　roughness　can　be　between　22　and　23 μm.　Only　balling　effect　due　to　spattering　and　a　small　amount　of　porosity　were　found　in　the　cross-section　of　CLTS.　The　relative　density　of　most　of　the　fabricated　parts　is　higher　than　99%,　and　the　highest　relative　density　is　up　to　99.99%.　IPLT　has　longer　and　thicker　martensite　than　LLTF,　and　the　β-grain　of　CLTS　is　also　coarser　than　LLTF.　The　tensile　properties　of　CLTS　are　similar　to　those　of　LLTF,　and　the　ultimate　tensile　strength,　yield　strength　and　elongation　of　CLTS　are　1205 MPa,　1107 MPa　and　6.8%,　respectively.　Due　to　the　anisotropy　of　the　LPBF,　the　horizontally　constructed　Ti–6Al–4 V　specimens　yielded　higher　strengths,　while　the　vertically　constructed　specimens　obtained　better　elongation.　The　fracture　of　the　part　is　characterized　by　a　mixture　of　brittle　fracture,　ductile　fracture　and　quasi-dissociative　fracture.　The　surface　quality,　relative　density　and　mechanical　properties　of　CLTS　are　similar　to　those　of　LLTF,　while　the　forming　efficiency　is　much　higher　than　that　of　LLTF,　which　can　reach　7.35　mm3/s.　Graphical　abstract:　[Figure　not　available:　see　fulltext.]　©　2023,　The　Author(s),　under　exclusive　licence　to　Springer-Verlag　London　Ltd.,　part　of　Springer　Nature.