Members　of　the　Numb-associated　kinase　family　of　serine/threonine　kinases　play　an　essential　role　in　many　cellular　processes,　such　as　endocytosis,　autophagy,　dendrite　morphogenesis,　osteoblast　differentiation,　and　the　regulation　of　the　Notch　pathway.　Numb-associated　kinases　have　been　relevant　to　diverse　diseases,　including　neuropathic　pain,　Parkinson’s　disease,　and　prostate　cancer.　Therefore,　they　are　considered　potential　therapeutic　targets.　In　addition,　it　is　reported　that　Numb-associated　kinases　have　been　involved　in　the　life　cycle　of　multiple　viruses　such　as　hepatitis　C　virus　(HCV),　Ebola　virus　(EBOV),　and　dengue　virus　(DENV).　Recently,　Coronavirus　disease　2019　(COVID-19)　caused　by　severe　acute　respiratory　syndrome　coronavirus　2　(SARS-CoV-2)　continues　to　threaten　global　health.　Studies　show　that　Numb-associated　kinases　are　implicated　in　the　infection　of　SARS-CoV-2　which　can　be　suppressed　by　Numb-associated　kinases　inhibitors.　Thus,　Numb-associated　kinases　are　proposed　as　potential　host　targets　for　broad-spectrum　antiviral　strategies.　We　will　focus　on　the　recent　advances　in　Numb-associated　kinases-related　cellular　functions　and　their　potential　as　host　targets　for　viral　infections　in　this　review.　Questions　that　remained　unknown　on　the　cellular　functions　of　Numb-associated　kinases　will　also　be　discussed.　©　2023,　The　Author(s),　under　exclusive　licence　to　Springer　Nature　B.V.