In　order　to　efficiently　utilize　the　waste　bituminous　pavement　materials　and　waste　bio-oil,　the　composition　of　bio-oil　rejuvenator　was　optimized.　The　rejuvenation　efficiency　of　the　waste　bio-oil　rejuvenator　was　evaluated　from　rheological　properties　and　chemical　composition.　In　addition,　a　quantitatively　evaluation　index　for　the　rejuvenation　efficiency　was　proposed.　The　results　show　that　the　central　composite　design　and　response　surface　methodology　is　effective　in　optimizing　composition　of　rejuvenators.　The　dosage　of　waste　bio-oil　in　the　rejuvenated　bitumen　reached　9%.　Compared　to　another　two　commercial　rejuvenators,　waste　bio-oil　rejuvenator　rejuvenated　the　aged　bitumen　more　effectively.　Its　rejuvenation　efficiency　for　rheological　parameters　of　aged　bitumen　was　close　to　100%.　The　waste　bio-oil　rejuvenator　restored　the　performance　of　aged　bitumen　by　reducing　the　proportion　of　macromolecular　substances.　The　rejuvenation　efficiency　of　aromatics,　colloidal　instability　index　and　carbonyl　functional　groups　were　57.1%,　126.9%　and　78.7%　respectively.