Biological　co-metabolism　is　an　economical　and　efficient　technique　for　treating　refractory　organic　matter,　and　in　recent　years,　it　has　been　widely　used　in　the　treatment　of　chlorophenol-containing　wastewater.　It　has　been　found　that　many　conditions　affect　the　bio　co-metabolism　efficiency,　such　as　the　carbon　source　type,　carbon　source　content,　microorganism　types,　and　environmental　factors.　The　carbon　source　concentration　experiment　showed　that　when　the　dosage　ratio　of　sodium　acetate　to　black　aniline　powder　was　1:2,　the　degradation　rate　of　black　aniline　powder　was　82%,　and　the　removal　rate　was　92.9%.　When　tetrachlorophenol　increased　from　210　mg/L　to　2100　mg/L,　the　tetrachlorophenol　was　increased　in　the　effluent,　and　the　microorganism＇s　activity　was　inhibited.　Besides,　the　sedimentation　performance　of　activated　sludge　was　also　damaged.　The　temperature　test　showed　that　the　removed　4-chlorophenol　was　as　high　as　2100　mg/L　at　35　C,　and　the　apparent　4-chlorophenol　residue　in　the　effluent　could　be　detected　at　20　C.　Therefore,　by　appropriately　controlling　the　external　operating　conditions　of　the　reactor,　the　co-metabolism　of　refractory　organic　such　as　chlorophenols　can　be　achieved.　　©　The　Authors,　published　by　EDP　Sciences.