Lung　cancer　is　one　of　the　most　lethal　malignant　tumors　in　the　world.　Non-small　cell　lung　cancer　(NSCLC)　is　the　most　common　pathological　subtype.　However,　the　molecular　mechanism　of　NSCLC　progress　is　still　unclear.　We　extracted　the　expression　data　of　the　Bruton＇s　tyrosine　kinase　(BTK)　gene　in　NSCLC　tissues　from　the　TCGA　database.　The　results　of　paired　t-test　showed　that　the　BTK　gene　was　significantly　underexpressed　in　NSCLC　tissues.　To　further　verify　the　above　results,　we　detected　the　expression　of　the　BTK　gene　in　NSCLC　cell　lines　A549,　H1299,　and　H1650　at　the　RNA　and　protein　levels　by　real-time　fluorescent　quantitative　polymerase　chain　reaction　and　Western　Blot　analysis,　respectively.　The　results　showed　that　BTK　was　low　expressed　in　NSCLC　tissues　and　cells.　More　importantly,　the　expression　of　the　BTK　gene　is　also　significantly　related　to　the　patient＇s　age,　gender,　tumor　range　(T),　lymph　node　invasion　(N),　tumor　stage,　and　prognosis,　and　its　expression　level　gradually　decreases　with　the　progress　of　the　disease.　It　is　speculated　that　BTK　may　be　an　independent　prognostic　factor　of　NSCLC.　Our　experimental　results　are　consistent　with　the　above　clinical　correlation　analysis　results.　Overexpression　of　BTK　can　significantly　inhibit　the　proliferation,　migration,　and　invasion　of　NSCLC　cells　and　can　block　the　G0/G1　tumor　cell　cycle,　indicating　that　overexpression　of　BTK　can　inhibit　the　growth,　migration,　and　invasion　of　NSCLC　cells.　©　2023　Wenjia　Ren　et　al.