A　sequencing　batch　reactor　(SBR)　was　used　to　start　the　completely　autotrophic　nitrogen　removal　over　nitrite　(CANON)　process.　The　effects　of　long-term　starvation　stress　and　short-term　starvation　regulation　on　nitrogen　removal　were　investigated.　The　activities　of　functional　bacteria　before　and　after　short-term　starvation　were　tested　to　analyze　the　inhibitory　effect　of　repeated　short-term　starvation　on　nitrite　oxidizing　bacteria　(NOB)　under　shortened　recovery　time.Microbial　sequencing　was　conducted　at　different　stages　of　CANON　initiation　process　to　analyze　changes　in　community　structure.　The　results　show　that　the　TN　removal　efficiency　(NRE)　of　the　system　can　reach　62.34%　when　the　system　is　restored　7days　after　chronic　starvation.　The　short-term　starvation-recovery　mode　can　accelerate　the　start-up　speed　of　the　process,　and　successfully　control　the　ΔNO3--N/ΔNH4+-N　in　SBR　between　0.11.　The　average　removal　rate　of　ammonia　removal　efficiency　(ARE)　exceeds　95%　and　the　average　NRE　reaches　79.45%.　After　short-term　starvation　regulation,　the　NOB　activity　decreased　from　91.32mgN/(gVSS·d)　to　45.40mgN/(gVSS·d),　and　increased　to　219.25mgN/(gVSS·d)　compared　with　the　anammox　activity　(SAA),　ensuring　the　CANON　nitrogen　removal　performance.　High-throughput　sequencing　has　shown　that　the　Aerobic　Ammonia-Oxidation　Bacteria　(AerAOB)　functional　bacterium　Nitrosomonas　can　recover　quickly　after　experiencing　long-term　starvation.　Candidatus　Kuenenia　has　the　ability　to　resist　long-term　starvation,　and　the　relative　abundance　of　Candidatus　Kuenenia　is　as　high　as　69.04%　after　7days　of　recovery.　The　relative　abundance　of　Candidatus　Kuenenia　decreased　in　the　stable　recovery,　but　reached　61.19%　after　the　process　was　started,　thus　achieving　the　enrichment　of　Anaerobic　Ammonia-Oxidation　Bacteria　(AnAOB).　The　CANON　process　was　able　to　recover　from　long-term　starvation　stress,　and　short-term　starvation　regulation　was　beneficial　for　process　efficiency.　©　2023　Chinese　Society　for　Environmental　Sciences.　All　rights　reserved.