Due　to　the　lightweight　nature　of　timber,　vibration　serviceability　is　a　crucial　issue　in　the　design　of　timber　floors.　The　purpose　of　this　study　is　to　investigate　how　beam–panel　connections　affect　the　vibration　serviceability　of　cross-laminated　timber　(CLT)　floors　subjected　to　multi-person　loading.　Cyclic　tests　were　carried　out　to　determine　the　mechanical　behaviour　of　steel　beam–CLT　panel　connections　with　various　screws　sizes　(diameters　and　lengths).　A　numerical　model　of　a　CLT　floor　was　developed　to　determine　the　response　to　human-induced　vibrations　with　different　screw　configurations　(sizes　and　spacing).　The　results　showed　that　the　dynamic　characteristics　of　the　floor　were　slightly　impacted　by　the　screw　size.　However,　as　the　spacing　between　screws　reduced,　the　fundamental　natural　frequency　increased　by　4.3%　and　the　vibration　dose　value　(VDV)　of　the　floor　decreased　by　38.3%.　A　theoretical　model　was　introduced　to　predict　the　fundamental　natural　frequency　of　a　CLT　floor　system.　In　addition,　a　design　method　for　predicting　the　vibration　serviceability,　in　terms　of　VDV,　of　low-frequency　CLT　floors　was　proposed.　©　2023　The　Authors