Background:　Although　functional　brain　connectivity　in　anxiety-related　disorders　has　been　studied,　brain　connectivity　in　non-clinical　populations　with　high　trait　anxiety　has　been　rarely　reported.　Whether　structural　brain　connectivity　changes　in　young　healthy　individuals　with　high　trait　anxiety　remains　unknown.　Methods:　Thirty-eight　young　healthy　individuals　with　high　anxiety　levels　and　34　healthy　subjects　with　low　anxiety　levels　who　were　matched　by　age,　gender,　and　educational　level　were　recruited.　Diffusion　tensor　images　were　acquired　to　analyze　white　matter　connectivity.　A　two-sample　t-test　was　used　for　group　comparison　of　weighted　networks　and　graph　properties.　Results:　Different　connections　were　detected　in　fractional　anisotropy-　and　fiber　number-weighted　networks.　These　connections　were　widely　distributed　in　various　regions,　where　relative　significance　was　located　in　the　inter-hemispheric　frontal　lobe,　the　frontal-limbic　lobe　in　the　right　intra-hemisphere,　and　the　frontal-temporal　lobe　in　the　ipsilateral　hemisphere.　However,　no　significant　difference　was　found　in　fiber　length-weighted　network　and　in　graph　properties　among　the　three　networks.　Conclusions:　The　structural　connectivity　of　white　matter　may　be　a　vulnerability　marker.　Hence,　healthy　individuals　with　high　trait　anxiety　levels　are　susceptible　to　anxiety-related　psychopathology.　The　findings　may　help　elucidate　the　pathological　mechanism　of　anxiety　and　establish　interventions　for　populations　susceptible　to　anxiety　disorders.