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Abstract:
大肠杆菌中具有产氢功能基因的载体pET32a‑fdhF‑1及其构建和应用,涉及基因工程技术领域。pET32a‑fdhF‑1基因载体为包含有大肠杆菌BL21甲酸脱氢酶fdhF基因的pET32a质粒。其构建是通过设计引物扩增大肠杆菌BL21的甲酸脱氢酶(formate dehydrogenase H)的fdhF基因片段,并通过T4 DNA连接酶将其连接到酶切后的pET32a载体中,命名为pET32a‑fdhF‑1,进行基因测序,验证基因的完整性。将pET32a‑fdhF‑1质粒转化到大肠杆菌中,在产氢培养基中进行培养产氢。转入载体pET32a‑fdhF‑1能提高大肠杆菌产氢效率。
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Patent Info :
Type: 发明申请
Patent No.: CN201810093583.5
Filing Date: 2018-01-31
Publication Date: 2018-06-29
Pub. No.: CN108220319A
Applicants: 北京工业大学
Legal Status: 实质审查 ; 复审
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ESI Highly Cited Papers on the List: 0 Unfold All
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30 Days PV: 10
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