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Author:

王海燕 (王海燕.) | 刘伟 (刘伟.) | 赵雅琪 (赵雅琪.) | 徐岚婷 (徐岚婷.) | 王冕超 (王冕超.)

Indexed by:

incoPat zhihuiya

Abstract:

大肠杆菌中具有产氢功能基因的载体pET32a‑fdhF‑1及其构建和应用,涉及基因工程技术领域。pET32a‑fdhF‑1基因载体为包含有大肠杆菌BL21甲酸脱氢酶fdhF基因的pET32a质粒。其构建是通过设计引物扩增大肠杆菌BL21的甲酸脱氢酶(formate dehydrogenase H)的fdhF基因片段,并通过T4 DNA连接酶将其连接到酶切后的pET32a载体中,命名为pET32a‑fdhF‑1,进行基因测序,验证基因的完整性。将pET32a‑fdhF‑1质粒转化到大肠杆菌中,在产氢培养基中进行培养产氢。转入载体pET32a‑fdhF‑1能提高大肠杆菌产氢效率。

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Patent Info :

Type: 发明申请

Patent No.: CN201810093583.5

Filing Date: 2018-01-31

Publication Date: 2018-06-29

Pub. No.: CN108220319A

Applicants: 北京工业大学

Legal Status: 实质审查 ; 复审

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 10

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