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Abstract:
一种针对膨胀污泥中Type0092型丝状菌群聚合酶链式反应方法,涉及污水生物处理领域。反应体系:2倍浓度的Taq PreMix试剂12.5μl;DNA模板0.5-1μl;正向引物U27f(10μm/L)0.5-1μl;反向引物U1492r(浓度为10μm/L)0.5-1μl;加灭菌水到总体积25μl。程序:①1个循环:温度为94℃时间为2-5min。②35个循环:依次为变性温度94℃,时间20-45s;复性温度53-55℃,时间30-45s;延伸温度72℃,时间1-2min。③1个循环:温度72℃,时间5-8min。将扩增产物在1.5%的琼脂糖凝胶电泳中跑胶,得到分离良好的DNA亮条带。本发明快速准确地对Type0092型丝状菌群进行PCR反应扩增,进行鉴定分析,为后续分析提供基础。
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Patent Info :
Type: 发明申请
Patent No.: CN201510267145.2
Filing Date: 2015-05-24
Publication Date: 2015-09-02
Pub. No.: CN104878000A
Applicants: 北京工业大学
Legal Status: 撤回-视为撤回
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WoS CC Cited Count: 0
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ESI Highly Cited Papers on the List: 0 Unfold All
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Chinese Cited Count:
30 Days PV: 8
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