In　this　paper,　a　method　for　stress　measurement　of　semi-infinite　structural　is　presented　based　on　line-focus　acoustic　microscopy.　Based　on　the　acoustoelastic　theory　and　the　superposition　of　partial　bulk　wave　(SPBW)　method,　the　theoretical　model　of　acoustoelastic　surface　waves　under　the　uniaxial　stress　state　is　established　and　the　effects　of　stress　on　the　surface　wave　are　investigated　for　the　isotropic　semi-infinite　structural.　Experimental　measurements　of　surface　wave　velocities　under　different　stress　conditions　are　carried　out　using　an　independently　developed　acoustic　microscopy　system　with　a　PVDF　line　focusing　probe,　and　the　relationship　between　stress　and　surface　wave　velocity　is　obtained.　Both　theoretical　predictions　and　experimental　results　indicate　that　tensile　stress　causes　a　reduction　in　surface　wave　velocity.　This　research　provides　a　powerful　technique　for　the　non-destructive　evaluation　of　thick　material.　Furthermore,　it　can　be　a　great　tool　for　the　evaluation　of　stress.　©　2024,　The　Author(s),　under　exclusive　license　to　Springer　Nature　Switzerland　AG.