This　paper　presents　a　multiple　speech　source　separation　method　that　adopts　the　minimum　variance　distortionless　response　(MVDR)　beamformer　to　separate　speech　sources　based　on　the　direction-of-arrival　(DOA)　estimated　from　the　B-format　microphone　recordings.　Unlike　conventional　microphone　arrays,　the　B-format　microphone　provides　complete　spatial　information　of　the　pressure　gradient　and　acoustic　pressure　which　is　useful　to　multiple　sources　localization.　Therefore,　this　paper　adopts　the　single-source　points　detection　of　the　B-format　microphone　recordings,　where　only　the　dominant　source　is　present　in　the　time-frequency　domain.　A　higher　accuracy　of　source　localization　is　achieved　by　identifying　and　utilizing　only　single-source　points　which　contain　correct　direction　cues.　According　to　the　DOA　estimates,　MVDR　beamformer　is　then　adopted　for　recovering　the　sources　separately.　The　objective　evaluation　shows　that　the　proposed　method　outperforms　the　existing　BSS　algorithm.　©　2020　ACM.