The　coupling　of　denitrifying　phosphorus　removal　and　Anammox　can　further　reduce　the　carbon　source　needed　for　nitrogen　and　phosphorus　removal,　and　the　stable　production　of　NO2--N　is　the　key　to　the　independent　and　joint　operation　of　the　two　processes.　Therefore,　with　low　C/N　domestic　sewage　as　the　research　object,　flocculent　sludge　and　a　small　amount　of　long-term　stored　aerobic　granular　sludge　were　inoculated　in　the　reactor.　The　short-cut　nitrification-denitrification-dephosphorization　granular　sludge　was　cultivated　in　60　days　by　two-stage　gradient　aeration　with　the　stop　aeration　time　(3min:　3min)　controlled,　and　the　system　denitrification　and　dephosphorization　performance　was　analyzed　in　this　process.　The　results　show　that　the　average　particle　size　of　granular　sludge　is　841μm　and　the　SVI　is　31.23ml/g　during　the　stable　period.　The　particle　structure　is　compact　and　the　settling　performance　is　good.　The　effluent　NO3--N　was　less　than　0.1mg/L,　TP　was　less　than　0.7mg/L,　NO2--N　was　more　than　15mg/L,　which　realized　the　efficient　removal　of　P　and　accumulation　of　NO2--N,　and　could　provide　a　stable　NO2--N　substrate　for　subsequent　coupling　of　Anammox.　The　results　of　batch　experiments　showed　that　57.63%　of　the　DPAOs　in　the　particles　which　could　use　NO2--N　as　electron　acceptor,　and　the　enrichment　of　DPAOs　improved　the　phosphorus　removal　ability　of　the　system.　High-frequency　intermittent　gradient　aeration　can　achieve　a　high　coupling　between　AOB　and　DPAOS,　but　it　is　difficult　to　achieve　NOB　suppression　only　by　intermittent　aeration.　Then,　by　adding　gradient　aeration　in　the　front　section　of　＂ammonia　trough　point＂　to　optimize　the　actual　oxygen　limiting　aeration　point,　the　inhibition　of　NOB　can　be　enhanced,　so　as　to　achieve　good　accumulation　of　nitrite.　©　2021,　Editorial　Board　of　China　Environmental　Science.　All　right　reserved.