Partial　Denitrification　coupled　with　Anammox　(PDA)　process　was　used　as　for　nitrogen　removal　from　wastewater.　However,　the　phosphate　removal　still　relies　on　chemical　methods　in　wastewater　treatment　plants.　Denitrifying　Phosphorus　Removal　(DPR)　process　allows　phosphorus　uptake　under　anoxic　conditions　using　nitrate　(NO3−)　as　electron　acceptor.　In　this　study,　the　PDA　and　DPR　processes　were　successfully　coupled　into　a　novel　integration　(DPR-PDA)　for　co-treatment　of　simulated　domestic　wastewater　and　NO3−　wastewater　to　achieve　simultaneous　nitrogen　and　phosphorus　removal.　The　effluent　TN　and　TP　were　0.9　and　0.2　mg/L　during　phase　Ⅵ　(day450-513)　under　influent　COD/TN　of　1.8,　respectively.　Anammox　bacteria　was　self-enriched　in　this　fully　suspended　sludge　system,　with　abundances　increasing　from　undetected　at　day19　to　2.0　×　1010　copies/g　dry　sludge　at　day468.　Anammox　bacteria　was　effectively　retained　and　was　responsible　for　the　main　nitrogen　removal,　accompanied　by　increasing　activity　up　to　3.1　mg　N/g　VSS/h.　Batch　tests　demonstrated　that　denitrifying　polyphosphate　accumulating　organisms　exhibited　DPR　capacity　immediately　after　the　introduction　of　NO3−,　but　not　after　the　introduction　of　nitrite　(NO2−)　only.　Candidatus_Competibacter　with　endogenous　denitrification　ability　was　enriched　from　13.30　%　to　35.42　%,　which　coexisted　with　anammox　bacteria　stably.　The　novel　DPR-PDA　process　is　a　promising　technology　for　simultaneous　removal　of　nitrogen　and　phosphorus　with　low　carbon　demand　and　without　aeration.　©　2023　Elsevier　B.V.