Digital　twin　is　currently　undergoing　a　significant　transformation　from　the　conceptual　and　theoretical　research　phase　to　the　implementation　and　application　phase.　However,　a　universally　adaptable　research　and　development　platform　for　digital　twin　is　critically　needed　to　meet　the　devel-opment　requirements.　Specifically,　a　publicly　accessible　simulation,　testing,　and　validation　platform　which　can　support　digital　twin　model　building,　data　processing,　algorithm　design,　configuration,　etc.,　is　urgently　required　for　researchers.　Furthermore,　for　developers　from　the　industry,　a　low-code　development　platform　that　can　offer　a　customizable　suite　of　functions　such　as　model　creation,　data　management,　protocol　configuration,　and　visualization　is　urgently　needed.　Meanwhile,　for　enterprise　users,　there　is　a　lack　of　an　application　management　platform　that　can　be　configured　and　migrated　for　various　application　scenarios,　functions,　and　modes.　Therefore,　based　on　the　sys-tem　research　of　digital　twin　theories　and　key　technologies　by　the　authors　(such　as　the　five-dimension　digital　twin　model,　digital　twin　modeling　and　digital　twin　data　theory,　digital　twin　standards,　and　so　on),　a　digital　twin　software　platform　reference　architecture,　namely　makeTwin,　is　proposed　and　designed,　as　well　as　its　ten　core　functions.　The　workflow　of　the　makeTwin　and　the　interaction　mechanisms　among　its　core　functions　are　described.　Finally,　a　digital　twin　application　system　for　a　chemical　fiber　textile　shop　floor　(CFTS)　which　was　developed　according　to　makeTwin,　is　introduced,　which　validates　the　proposed　reference　architecture.(c)　2023　Production　and　hosting　by　Elsevier　Ltd.　on　behalf　of　Chinese　Society　of　Aeronautics　and　Astronautics.　on　behalf　of　Chinese　Society　of　Aeronautics　and　Astronautics.This　is　an　open　access　article　under　the　CC　BY-NC-ND　license　(http://creativecommons.org/licenses/by-nc-nd/4.0/).