Continuous　flow　processes　for　the　in　situ　determination　of　N2O　emissions　during　low　C/N　municipal　wastewater　treatment　have　rarely　been　reported.　The　anaerobic/aerobic/anoxic　(AOA)　process　has　recently　shown　promising　potential　in　energy　savings　and　advanced　nitrogen　removal,　but　it　still　needs　to　be　comprehensively　explored　in　relation　to　N2O　emissions　for　its　carbon　reduction　advantages.　In　this　study,　a　novel　gas-collecting　continuous　flow　reactor　was　designed　to　comprehensively　evaluate　the　emissions　of　N2O　from　the　gas　and　liquid　phases　of　the　AOA　process.　Additionally,　the　measures　of　enhancing　endogenous　denitrification　(ED)　and　self-enriching　anaerobic　ammonium　oxidation　(Anammox)　were　employed　to　optimize　nitrogen　removal　and　achieve　N2O　reduction　in　the　anoxic　zone.　The　results　showed　that　enhanced　ED　coupled　with　Anammox　led　to　an　increase　in　the　nitrogen　removal　efficiency　(NRE)　from　67.65　to　81.96%,　an　enhancement　of　the　NO3-　removal　rate　from　1.76　mgN/(L　h)　to　3.99　mgN/(L　h),　and　the　N2O　emission　factor　in　the　anoxic　zone　decreased　from　0.28　to　0.06%.　Impressively,　ED　eliminated　91.46　±　2.47%　of　the　dissolved　N2O　from　the　upstream　aerobic　zone,　and　the　dissolved　N2O　in　the　effluent　was　reduced　to　less　than　0.01　mg/L.　This　study　provides　valuable　strategies　for　fully　evaluating　N2O　emissions　and　N2O　reduction　from　the　AOA　process.　©　2024　American　Chemical　Society.