The　existence　of　antibiotic　resistant　bacteria　(ARB)　and　antibiotic　resistance　genes　(ARGs)　has　been　a　global　public　environment　and　health　issue.　Due　to　the　different　cell　structures,　gram-positive/negative　ARB　exhibit　various　inactivation　mechanisms　in　water　disinfection.　In　this　study,　a　gram-negative　ARB　Escherichia　coli　DH5α　(E.　coli　DH5α)　was　used　as　a　horizontal　gene　transfer　(HGT)　donor,　while　a　gram-positive　ARB　Bacillus　as　a　recipient.　To　develop　an　efficient　and　engineering　applicable　method　in　water　disinfection,　ARB　and　ARGs　removal　efficiency　of　Fe(VI)　coupled　peroxydisulfate　(PDS)　or　peroxymonosulfate　(PMS)　was　compared,　wherein　hydroxylamine　(HA)　was　added　as　a　reducing　agent.　The　results　indicated　that　Fe(VI)/PMS/HA　showed　higher　disinfection　efficiency　than　Fe(VI)/PDS/HA.　When　the　concentration　of　each　Fe(VI),　PMS,　HA　was　0.48　mM,　5.15　log　E.　coli　DH5α　and　3.57　log　Bacillus　lost　cultivability,　while　the　proportion　of　recovered　cells　was　0.0017　%　and　0.0566　%,　respectively,　and　HGT　was　blocked.　Intracellular　tetA　was　reduced　by　2.49　log.　Fe(IV)　and/or　Fe(V)　were　proved　to　be　the　decisive　reactive　species.　Due　to　the　superiority　of　low　cost　as　well　as　high　efficiency　and　practicality,　Fe(VI)/PMS/HA　has　significant　application　potential　in　ARB,　ARGs　removal　and　HGT　inhibition,　offering　a　new　insight　for　wastewater　treatment.　©　2024　Elsevier　B.V.