The　efficient　degradation　of　antibiotics　holds　significant　implications　for　mitigating　environmental　pollution.　This　study　synthesized　a　montmorillonite　chitosan　composite　material　(MMT-CS)　using　the　gel　template　method.　Subsequently,　a　bio-enhanced　reactor　was　constructed　to　facilitate　the　degradation　of　chlorotetracycline　(CTC).　The　addition　of　MMT-CS　composite　material　enables　the　degradation　of　different　concentrations　of　CTC.　MMTCS,　a　conductive　carrier,　effectively　promotes　microbial　adhesion　and　boosts　the　metabolic　activity　of　functional　microorganisms.　Additionally,　it　facilitates　the　maintenance　of　microbial　activity　under　CTC　pressure　by　promoting　the　secretion　of　extracellular　polymeric　substances,　increasing　critical　enzyme　activity,　and　enhancing　the　electron　transfer　capacity　within　the　system.　In　this　MMT-CS　bio-enhanced　process,　Paracoccus　(11.4%)　and　Bacillus　(3.9%)　are　utilized　as　essential　bacteria　genes.　The　results　of　metabolic　pathways　prediction　indicated　significant　enhancements　in　membrane-transport,　nucleotide-metabolism,　replication-repair,　and　lipidmetabolism.　Thus,　the　developed　self-supporting　MMT-CS　bio-enhanced　process　ensured　the　stability　of　the　system　during　the　removal　of　antibiotics.