Clay-based　2D　nanofiltrations　(NFs)　have　shown　great　potentials　in　wastewater　treatment　owing　to　their　facile　scale-up　and　low-cost　feature.　However,　the　swelling　effect　seriously　limits　their　separation　efficient　towards　the　construction　of　high-performance　NFs.　Herein,　an　anti-swelling　clay-based　2D　NF　is　developed　based　on　the　strategy　of　electrostatic　interlamellar　fixation.　The　intercalation　of　positively-charged　Fe(OH)(3)　nanoparticles　could　effectively　fix　the　interlayer　spacing　of　negatively-charged　montmorillonite-based　2D　NF,　contributing　to　a　high　dye　(Congo　red)　retention　rate　up　to　99.78　%　and　also　a　satisfactory　flux　of　60.65　L　m(-2)　h(-1)　bar(-1).　Separation　mechanism　was　explored　for　the　combined　effects　of　size,　electrostatic　potential　and　conjugation　on　the　retention　rate.　When　scaling　up　(similar　to　700　cm(2)),　the　anti-swelling　2D　NF　still　maintain　an　excellent　separation　effect　that　is　superior　to　almost　all　reported　state-of-the-art　2D　NFs.　According　to　the　detailed　life　cycle　assessments　(LCA),　the　advantages　of　MMT　in　the　production　process　were　demonstrated.　The　utilization　of　2D　clay　material　also　exhibits　superior　resource,　environmental　and　techno-economic　benefits　compared　to　other　mainstream　2D　materials.　This　work　proposes　an　effective　strategy　to　solve　the　swelling　effect　of　clay-based　2D　NFs　towards　the　large-scale　utilization　of　high-performance　and　low-cost　separation　membrane　for　efficient　wastewater　treatment.