Fungi　capable　of　simultaneous　nitrogen　and　phosphorus　removal　from　wastewater　is　rarely　found.　Here,　a　novel　fungal　strain　(SNDM1)　performing　heterotrophic　nitrification,　aerobic　denitrification,　and　phosphate　removal　was　isolated　and　identified　as　Mucor　circinelloides.　The　favorable　nutrient　removal　conditions　by　the　strain　using　glucose　were　C/N　ratios　of　25–30,　salinities　of　0　%–3　%,　and　pH　of　7.5.　Strain　SNDM1　achieved　ammonium,　nitrite,　nitrate,　and　phosphate　removal　rates　of　5.23,　10.08,　4.88,　and　0.97　mg/L/h.　Nitrogen　balance　indicated　that　gaseous　(18.60　%–24.55　%)　and　intracellular　nitrogen　(43.76　%–70.63　%)　were　primary　fate　of　initial　nitrogen.　Enzyme　activity　revealed　that　ammonium　removal　occurred　through　heterotrophic　nitrification　and　aerobic　denitrification.　Removed　phosphorus　was　mainly　transformed　into　cell　membranes　(56　%–64　%)　and　extracellular　polymeric　substances　(20　%–26　%).　Orthophosphate　was　the　major　intracellular　phosphorus　species,　while　polyphosphate　and　pyrophosphate　existed　extracellularly.　These　findings　highlight　the　potential　of　this　fungal　strain　for　bioremediating　polluted　wastewater.　©　2024　Elsevier　Ltd