Achieving　a　high-density,　repeatable,　and　uniform　distribution　of　＂hotspots＂　across　the　entire　surface-enhanced　Raman　scattering　(SERS)　substrate　is　a　current　challenge　in　facilitating　the　efficient　preparation　of　large-area　SERS　substrates.　In　this　study,　we　aim　to　produce　homogeneous　surface-enhanced　Raman　scattering　(SERS)　substrates　based　on　the　strong　interaction　between　femtosecond　laser　pulses　and　a　thin　film　of　colloidal　gold　nanoparticles　(AuNPs).　The　SERS　substrate　we　obtained　consists　of　irregularly　shaped　and　sharp-edged　gold　nanoparticle　aggregates　with　specially　extruding　features;　meanwhile,　a　large　number　of　three-dimensional　AuNP　stacks　are　produced.　The　advantages　of　such　configurations　lie　in　the　production　of　a　high　density　of　hotspots,　which　can　significantly　improve　the　SERS　performance.　When　the　laser　fluence　is　5.6　mJ/cm(2),　the　substrate　exhibits　the　best　SERS　enhancement　effect,　and　a　strong　SERS　signal　can　still　be　observed　when　testing　the　concentration　of　R6G　at　10(-8)　mol/L.　The　enhancement　factor　of　such　SERS　substrates　prepared　using　femtosecond　laser　direct　writing　is　increased　by　3　orders　of　magnitude　compared　to　the　conventional　furnace　annealing　process.　Furthermore,　the　relative　standard　deviation　for　the　intensities　of　the　SERS　signals　was　measured　to　be　5.1%　over　an　area　of　50　x　50　mu　m(2),　indicating　a　highly　homogeneous　SERS　performance　and　excellent　potential　for　practical　applications.