This　study　evaluated　the　effectiveness　of　embedding　immobilization　technology　in　wastewater　treatment　and　its　capacity　to　enhance　the　hydrolysis　acidification　process.　Based　on　this　technology,　a　stable　anaerobic　environment　has　been　maintained.　Results　showed　that　the　rates　of　dissolved　organic　nitrogen　(DON)　and　dissolved　organic　phosphorus　(DOP)　conversion　both　exceeded　98　%　under　short　hydraulic　retention　time　(HRT　=　2h)　and　ambient　temperature.　Notably,　acetic　acid　and　propionic　acid　comprised　up　to　90.9　%　of　the　total　volatile　fatty　acids　in　the　effluent,　providing　suitable　carbon　sources　for　downstream　denitrification.　16S　rRNA　gene　sequencing　indicated　that　biofillers　effectively　enriched　and　retained　functional　bacteria,　causing　norank_Anaerolineaceae　(11.6　%-29.7　%)　and　norank_Bacteroidetes_vadinHA17　(10.8　%-14.9　%)　as　the　dominant　genera　in　the　reactor,　which　were　crucial　for　refractory　organic　matter　degradation.　Immobilized　biofillers　effectively　improved　wastewater　biodegradability,　supporting　a　stable　microbial　community　with　high　DON　and　DOP　conversion　rates　as　well　as　increased　VFA　accumulation.　©　2024