The　detection　of　exosomes　is　of　great　significance　for　early　diagnosis　and　treatment　of　cancer.　In　this　study,　based　on　the　-interaction　of　the　adenine　bases　of　DNA　with　graphene,　triple-stranded　DNA　(tsDNA)　was　assembled　on　the　surface　of　electrochemically　reduced　graphene　oxide　(ERGO)　to　obtain　a　tripod　DNA　probe.　The　exosomes　was　specifically　captured　by　exosome　membrane　protein　(CD63)　aptamer　(apt)　sequence　at　the　top　of　tsDNA,　and　the　introduction　of　hemin　effectively　enhanced　the　signal　intensity　of　the　Luminol-hydrogen　peroxide　(H2O2)　electrochemiluminescence　(ECL)　system.　The　ECL　intensity　of　the　biosensor　showed　a　good　linear　relationship　with　logarithm　of　exosome　concentration　in　the　concnetrtion　range　of　70-1.4x10　degrees　particles/mu　L,　and　the　detection　limit　was　30　particles/mu　L　(S/N=3).　This　tripod-structured　DNA　probe　facilitated　the　capture　and　detection　of　larger　targets,　and　was　expected　to　be　applied　to　the　analysis　of　actual　clinical　samples