Due　to　the　insufficient　detecting　method　of　the　arsenic　(As)　in　glass　beads　used　for　pavement　marking,　this　paper　validated　for　the　first　time　the　feasibility　of　hydride　generation　high-resolution　continuous　light　source　atomic　absorption　spectrometry　(HG-HRCS-AAS)　for　the　detection　of　As　in　glass　beads　and　proposed　a　simple,　sensitive,　and　accurate　two-factor　ANOVA　for　the　determination　of　the　As　in　glass　beads　by　HG-HRCS-AAS.　Through　the　analysis　of　the　orthogonal　experiment,　the　sample　pretreatments　were　optimized,　including　digestion　solution　composition,　reaction　temperature　and　time,　and　condensed　temperature,　to　achieve　the　requirements　of　glass　bead　digestion.　The　two-factor　ANOVA　was　applied　to　optimize　the　sample　detection　and　improve　the　measurement　accuracy　of　the　matrix　elements.　After　the　optimization,　the　relative　standard　deviation　(RSD)　was　in　the　range　of　0.75–2.47　%　for　the　As　determination.　The　optimized　detection　method　was　used　to　analyze　As　standard　material　with　a　recovery　rate　being　96–103　%.　The　limit　of　detection　(LOD)　and　limit　of　quantification　limit　(LOQ)　of　the　As　were　0.185　μg/L　and　0.617　μg/L,　respectively.　Therefore,　the　detection　method　can　be　successfully　applied　to　the　analysis　of　As　in　glass　beads　and　further　can　be　generalized　for　other　similar　materials.　©　2024　Elsevier　Ltd