Random　lasers　(RLs)　with　a　simple　structure　and　low-cost　properties　have　been　recognized　as　an　ideal　analytical　platform　and　are　still　challenging　for　liquid　detecting,　remaining　beset　for　low　sensitivity,　complicated　operation,　and　large　analyte　consumption.　Here,　inspired　by　a　microfluidic　sensor,　a　microtubule　structured　random　laser　for　multifunctional　sensing　is　demonstrated.　The　random　laser　is　achieved　resorting　to　a　curly　PMMA　film　with　gain　and　scatterers　embedded　in　it.　By　coupling　the　high-order　WGM　with　a　weak　random　scattering　mode,　a　coherent　random　laser　with　a　low　threshold　of　0.62　MW　cm−2　and　a　high　Q　factor　of　4700　is　obtained.　The　sensing　process　has　been　demonstrated　based　on　two　kinds　of　representative　analytes　of　sucrose　solution　and　TiO2　NP　suspension,　which　respond　to　the　variation　of　gain　and　scattering.　The　RL　sensor　features　fast　detection,　easy　operation,　and　low　cost,　which　may　provide　a　new　approach　for　their　further　applications　in　analytical　microfluidic　chip　and　disposable/pocket　analytical　instruments.　©　2025　Optica　Publishing　Group.　All　rights,　including　for　text　and　data　mining　(TDM),　Artificial　Intelligence　(AI)　training,　and　similar　technologies,　are　reserved.