The　sulfur-driven　autotrophic　partial　denitrification　coupled　with　anammox　(SPDA)　process　showed　significant　advantages　in　energy　conservation　and　resource　recovery　in　municipal　wastewater　treatment.　However,　its　application　in　regions　with　seasonal　temperature　fluctuations　and　high　latitudes　is　challenged　by　low　temperatures.　In　this　study,　the　feasibility　of　the　SPDA　process　for　treating　low-strength　municipal　wastewater　across　a　wide　temperature　range　(30–10　°C)　was　systematically　investigated.　The　results　demonstrated　that　thiosulfate-driven　autotrophic　partial　denitrification　maintained　an　efficient　nitrate　removal　rate　of　7.82　mg　NO3--N/gVSS/h　and　a　nitrate　to　nitrite　transformation　rate　of　62.7　%　even　at　temperatures　as　low　as　10　°C.　Molecular　ecological　network　and　DNA-SIP　revealed　that　dominant　sulfur-oxidizing　bacteria　(SOB)　shifted　from　norank_f_Hydrogenophilaceae　and　Thiobacillus　at　higher　temperatures　(30–20　°C)　to　Thiobacillus　and　Sulfurimonas　as　temperature　decreased,　thus　ensuring　the　performance　of　autotrophic　partial　denitrification　and　consistent　nitrite　supply　for　anammox.　Metagenomic　analysis　showed　that　the　abundance　of　functional　genes　related　to　sulfur　conversion　increased　almost　universally,　ensuring　a　stable　electron　supply　for　nitrate　reduction　through　sulfur　oxidation　at　low　temperatures.　The　functional　genes　responsible　for　nitrate　reduction　changed　from　nar　genes　at　higher　temperatures　to　nap　genes　at　lower　temperatures,　while　a　decrease　in　the　abundance　of　hzs　and　hdh　genes　corresponding　to　reduced　anammox　performance.　This　study　highlights　the　stable　performance　of　the　sulfur-driven　autotrophic　denitrification　at　low　temperatures　and　the　reliability　of　coupling　with　anammox,　extending　the　applicability　of　SPDA　to　a　broader　geographical　range.　©　2025