The　aim　of　this　study　was　to　determine　a　compatible　method　for　co-production　of　fucoxanthin　and　eicosapentaenoic　acid　of　diatom　Nitzschia　laevis　by　mixotrophic　and　heterotrophic　cultivation　modes　in　view　of　cell　growth,　targeting　products＇　contents,　photosynthesis-related　characteristics　and　carbon　partitioning.　The　results　showed　that　mixotrophic　mode　enhanced　fucoxanthin　and　eicosapentaenoic　acid　yields　by　increasing　their　precursors　of　pyruvate　and　acetyl-CoA　at　the　expense　of　starch.　The　increase　of　chlorophylls　and　glyceraldehyde　3-phosphate　indicated　the　development　of　Calvin　cycle　and　carbon　repartitioning　in　mixotrophic　mode.　Consequently,　microalgal　cells　in　mixotrophic　mode　achieved　much　higher　fucoxanthin　(60.12%)　and　eicosapentaenoic　acid　(50.67%)　contents,　and　lower　starch　content　(30.2%)　compared　with　heterotrophic　mode.　Furthermore,　fucoxanthin　content　was　positively　correlated　with　eicosapentaenoic　acid　content　(adjusted　R-2　=　0.96).　Taken　together,　these　results　showed　that　the　mixotrophic　mode　could　be　a　promising　approach　for　the　co-production　of　fucoxanthin　and　eicosapentaenoic　acid　by　Nitzschia　laevis.