TiO2　3DOM　materials　were　prepared　by　infiltrating　TiO2　sol　synthesized　by　sol-gel　process　into　the　polystyrene　(PS)　colloidal　crystal　templates,　and　then　by　heating　treatment.　Obtained　3DOM　samples　were　characterized　by　scanning　electron　microscope　(SEM),　transmission　electron　microscope　(TEM),　energy　dispersive　spectrometry　(EDS),　and　infrared　spectra　(IR).　Results　show　that　the　pore　structure　of　3DOM　can　be　tuned　by　using　different　heating　temperature　and　different　size　of　PS　spheres.　This　paper　also　focuses　on　the　investigation　of　surface　characteristics　of　pore　wall　of　TiO2　3DOM.