Wound　infections　can　cause　inflammation　and　delay　healing,　which　becomes　an　important　obstacle　to　wound　recovery.　To　overcome　this　issue,　various　antibacterial　agents　have　been　integrated　into　wound　dressings　to　prevent　infection.　Silver　nanoparticles　(AgNPs)　are　promising　candidates　due　to　their　broad-spectrum　antibacterial　activities　and　no　drug　resistance.　In　this　study,　Dio-AgNPs　were　initially　obtained　by　biological　synthesis　using　the　flavonoid　compound　diosmetin　(Dio)　as　a　reducing　and　capping　agent.　Dio-AgNPs　exhibited　strong　antibacterial　activity　against　S.　aureus　and　E.　coli　by　destroying　the　bacterial　membrane　structure　and　inducing　the　production　of　reactive　oxygen　species　(ROS),　finally　leading　to　bacterial　death.　Furthermore,　the　composite　sponges　(SP-1,　SP-2,　and　SP-3)　for　preventing　wound　infection　were　formulated　using　chitosan　(CS)　and　polyvinyl　alcohol　(PVA)　with　different　concentrations　of　Dio-AgNPs　incorporated.　The　prepared　sponges　exhibited　interconnected　porous　structures　with　water　absorption　capacities　of　＞33　times　their　own　weight.　The　wound　healing　experiments　showed　that　after　14　days,　the　SP-3　sponge　promoted　complete　wound　healing　by　preventing　wound　infection,　which　is　comparable　to　the　commercial　AgNPs　gauze　materials.　SP-3　sponge　also　showed　good　biosafety.　This　work　prepared　a　novel　SP-3　sponge,　which　offers　an　effective　and　safe　alternative　for　treating　wound　infections.　©　2025　Elsevier　B.V.