A　titanium　alloy　is　widely　used　in　implants　for　its　excellent　mechanical　properties　and　corrosion　resistance.　However,　the　bonding　strength　between　a　titanium　alloy　and　bone　tissue　is　low,　and　the　bacterial　adhesion　is　easily　triggered　on　the　implant　surface,　which　may　cause　the　failure　of　implants.　Therefore,　surface　modification　is　necessary　to　improve　the　biological　activity　and　antibacterial　properties.　In　this　work,　four　different　types　of　laser-induced　periodic　surface　structure　(LIPSS)　surfaces　are　designed　and　fabricated　on　the　TiNi　alloy　by　a　femtosecond　laser　according　to　the　size　of　MC3T3-E1　mouse　embryonic　osteoblasts.　The　in　vitro　osteogenic　activity　of　the　LIPSS　surface　is　investigated.　It　is　found　that　the　LIPSS　helps　improve　the　in　vitro　osteogenic　activity,　and　bonelike　apatite　tends　to　deposit　and　distribute　on　the　LIPSS.　The　biological　activity　and　antibacterial　activity　of　the　LIPSS　surface　are　evaluated　through　cell　culture　experiments　and　Escherichia　coli　culture　experiments.　It　is　demonstrated　that　the　horizontal　LIPSS　sample　with　a　width　of　30　μm　has　the　highest　cell　proliferation　rate　(142.5%　after　1　day,　132.3%　after　3　days)　and　a　good　antibacterial　rate　(50.2%).　These　results　provide　guidance　for　the　application　of　the　LIPSS　in　biocompatibility　and　antibacterial　aspects.　©　2025　American　Chemical　Society.