Nano-polypyrrole　(PPy)　were　prepared　on　glassy　carbon　(GC)　electrodes　by　electrodeposited　method.　The　morphology　of　the　PPy　was　obtained　by　scanning　electron　microscopy　(SEM).　The　GOD/PPy/GC　electrode　was　prepared　by　immobilization　glucose　oxidase　(GOD)　on　the　PPy.　The　electrocatalytic　properties　of　GOD/PPy/GC　electrode　were　characterized　by　cyclic　voltograme　method;　the　results　showed　that　GOD　can　be　immobilized　on　the　nano-PPy　and　the　biological　activation　of　GOD　was　retained.　The　GOD/PPy/GC　electrode　exhibits　good　eleccatalytic　performance　without　any　electron　mediator　at　the　medium　of　phosphate　buffer　(0.1　mol/L).