In　this　paper　we　demonstrate　that　new　phase　defects　of　the　Gross-Pitaevskii　equation　(GPE)　can　be　produced　as　a　Aharonov-Bohm　effect　resulting　from　pure　phase　twist　injection　on　existing　defects.　This　is　a　phenomenon　that　has　physical　justification　in　the　hydrodynamic　interpretation　of　GPE.　Here　we　give　an　analytical　proof　of　its　effects　by　using　Fermi-Walker　transport　and　Biot-Savart　induction　law.　An　analytical　derivation　of　the　dispersion　relation　is　derived　from　the　superposition　of　phase　twist　on　the　fundamental　state.　Since　the　extra　twist　corresponds　to　a　topological　change　of　the　total　linking　number　of　the　system,　we　show　that　the　production　of　new　defects　is　just　another　manifestation　of　the　Aharonov-Bohm　effect.　We　propose　a　laboratory　experiment　for　Bose-Einstein　condensates　to　test　this　phenomenon　and　to　show　that　it　can　have　useful　applications　in　science　and　technology.