The　environmental　scanning　electron　microscope　(ESEM)　allows　the　examination　of　thermo-sensitive　hydrogels　in　their　natural　state　without　the　need　for　coating　or　drying.　The　observation　parameters　can　be　tailored　to　observe　the　porosity　and　true　microstructure　of　the　thermo-sensitive　hydrogels.　It　was　shown　in-situ　and　dynamic　process　that　the　present　of　the　thermo-sensitive　hydrogels　was　deswelling　with　changing　the　temperature　and　pressure　parameters　in　the　range　of　2°C　-　50°C　and　300　Pa　-　600　Pa.