This　study　was　aimed　to　evaluate　the　fatigue　characteristics　of　asphalt　binders　modified　with　different　dosages　of　bio-oil　under　a　variety　of　aging　conditions.　The　frequency　sweep　test　was　performed　to　determine　four　rheological　parameters　indicative　of　cracking　resistance.　The　linear　amplitude　sweep　(LAS)　test　was　conducted　to　yield　damage　characteristic　relationship,　failure　criterion,　and　failure　envelope　based　on　the　viscoelastic　continuum　damage　theory.　In　general,　consistent　observations　were　obtained　between　the　rheological　parameters　and　the　LAS-based　fatigue　analysis,　in　that　higher　percentages　of　bio-oil　in　modification　provided　improved　resistance　to　cracking,　while　higher　aging　intensities　produced　lower　fatigue　performance.　(C)　2019　Elsevier　Ltd.　All　rights　reserved.