By　using　the　general　purpose　finite　element　code　such　as　ANSYS,　It　is　not　easy　to　perform　spectrum　analysis　for　engineering　structure　when　spatially　varying　ground　motions　are　of　interest.　To　overcome　this　difficulty,　this　paper　presents　a　response　spectrum　analysis　of　high-pier　and　long-span　railway　bridges　subjected　to　multiple　support　excitations　based　on　stochastic　vibration　method.　The　selfcode　is　developed　by　using　Matlab　and　APDL　language　in　ANSYS.　Along-span　and　high-pier　bridge　is　employed　for　a　typical　numerical　example　and　the　multiple　support　excitation　analysis　of　this　railway　bridge　is　conducted.　The　influences　of　coherence　loss　effect,　wave-passage　effect　and　site-response　effect　are　considered.　The　results　indicate　that　the　proposed　approach　can　conveniently　consider　the　ground　motion　spatial　variations　in　the　spectrum　analysis.　©　2017　Taylor　&　Francis　Group,　London.