A　pilot-scale　bio-trickling　filter　(BTF)　packed　with　ceramsite　was　investigated　to　treat　butan-2-yl　ethanoate　(BE),　which　is　discharged　from　the　pharmaceutical　industry.　To　choose　an　appropriate　operation　technology,　the　performance　of　twin　bench-scale　BTFs,　vertical　(BTF1)　and　horizontal　(BTF2)　towers,　to　purify　BE　were　tested.　The　BTF1　had　superior　degradation　capabilities　when　compared　to　BTF2,　a　quick　start　up　of　three　days,　a　maximum　elimination　capacity　of　548.5　g/(m(3)h)　and　an　average　removal　efficiency　of　approximately　95%　with　an　empty　bed　residence　time　(EBRT)　of　17　s.　Therefore,　in　a　subsequent　pilot　test,　a　vertical　BTF　with　a　high　temperature　shade　net　was　setup,　which　demonstrated　effective　BE　degradation　performance.　The　pressure　drop　of　the　vertical　BTFs　could　be　addressed　effectively　by　commutation　and　optimizing　the　nutrient　supply.　A　high-throughput　sequencing　comparison　of　the　three　towers　indicated　similarity　in　the　microorganisms　between　the　two　vertical　BTFs,　indicating　that　the　flow　patterns　within　the　tower　could　affect　microorganisms　cultivation.