tetherin　cDNA　was　amplified　from　total　RNA　prepared　from　HeLa　cells　by　RT-PCR,　then　cloned　into　the　eukaryotic　expression　vector　pEGFP-C3.　The　generated　pEGFP-Tetherin　construct　was　transfected　into　COS-1　to　determine　the　expression　and　the　subcellular　localization　of　GFP-Tetherin　fusion　protein.　Under　CLSM　the　localization　of　GFP-Tetherin　fusion　protein　distributed　outside　the　nucleus　and　co-localized　with　TGN　in　COS-1　cells.　©　2010　IEEE.