The　high　prevalence　of　influenza　A　virus　is　identified　in　Hunan　Province　because　of　the　high　density　of　poultry　farms.　To　survey　the　variations　of　H9N2　subtype　avian　influenza　virus　in　Hunan　province,　we　analyzed　HA　and　NA　genes　of　10　virus　strains　isolated　from　different　areas　of　Hunan　Province.　All　these　strains　belong　to　the　Eurasian　lineage,　Y280-like　sub-lineage.　The　cleavage　sites　in　their　HA　genes　were　all　RSSR↓GLT,　corresponding　to　the　feature　of　low　pathogenic　AIV.　All　strains　had　an　L　(Leu)　at　the　site　234　in　the　HA　genes,　indicating　the　ability　of　binding　with　the　SAα-2,6　receptor.　NA　gene　stalk　deletions　at　aa　63-65　were　also　detected　from　all　the　isolates,　indicating　a　possibility　of　increased　virus　replication　in　mammals.　Our　findings　suggest　that　more　attention　should　be　paid　to　the　surveillance　of　H9N2　influenza　virus　and　its　direction　of　reassortment.　©　2018,　Science　Press.　All　right　reserved.