A　quick　process　was　proposed　to　screen　PAOs　based　on　the　aerobic/anaerobic　cultivation.　15　strains,　which　had　over　60%　phosphate　removal　rate,　were　screened　out　and　then　tested　by　16S　rDNA　and　biochemical　analyses.　Except　2　strains　of　Bacillus　sp.,　the　other　strains　were　γ-Proteobacteria,　mainly　Klebsiella　sp.　and　Pseudomonas　sp..　The　phosphate　removal　experiment　indicated　that　the　pH　values　at　the　end　of　fermentation　increased　with　the　increase　in　the　removal　capacity　of　the　PAOs.　The　phosphate　removal　rate　increased　from　52%　to　73.3%　after　adding　the　PAOs　strain　screened　into　active　sludge.　The　addition　of　the　PAOs　strain　was　an　effective　way　to　enhance　the　phosphate　removal　capacity　of　activated　sludge.