Aim.　P-glycoprotein,　the　product　of　the　ATP-binding　cassette　subfamily　B　member　1　(ABCB1)　gene　(or　the　so-called　multidrug　resistance　1　gene),　is　an　ATP-driven　efflux　pump　contributing　to　the　pharmacokinetics　as　well　as　the　pharmacokinetics　of　drugs　that　are　P-glycoprotein　substrates,　such　as　tacrolimus.　This　paper　describes　the　development　of　a　new　method　for　detection　of　the　3435C/T　and　2677G/T/A　single　nucleotide　polymorphisms　of　the　ABCB1　gene.　The　method　is　a　simple　sequence-specific　primer　polymerase　chain　reaction　(SSP-PCR).　Methods.　158　Chinese　health　checkup　examinees　and　214　transplant　recipients　were　included　in　the　study.　Genomic　DNA　was　extracted　from　peripheral　blood　and　amplified　with　SSP-PCR　to　detect　the　3435C/T　and　2677G/T/A　mutations　in　ABCB1.　The　SSP-PCR　condition　was　optimized,　and　the　PCR　results　were　compared　with　those　of　DNA　sequencing.　Results.　In　the　optimized　condition,　the　two　polymorphisms　could　be　clearly　distinguished　after　one-step　PCR　and　electrophoresis.　The　ABCB1　3435C/T　and　2677G/T/A　genotypes　of　the　subjects　were　scanned,　and　allele-specific　bands　were　successfully　amplified　by　SSP-PCR,　which　were　in　full　accordance　with　the　results　of　sequencing.　Conclusion.　As　a　fast,　simple　and　inexpensive　genotyping　tool,　the　method　would　be　practicable　in　large　clinical　studies　on　interindividual　pharmacokinetics.