Ethyl-bridged　organic-inorganic　hybrid　silica　particles　were　prepared　via　a　sol-gel　and　hydrothermal　synthesis　approach　using　1,2-bis(triethoxysilyl)ethane　(BTESE)　as　the　sole　precursor,　and　triblock　copolymer　poly(ethylene　oxide)-block-poly(propylene　oxide)-block-poly(ethylene　oxide)　(P123)　and　dodecyltrimethylammonium　bromide　(DTAB)　as　combined　templates.　The　morphology,　pore　structure,　chemical　composition　and　liquid　chromatographic　performance　of　the　obtained　materials　were　investigated　in　detail.　The　particles　exhibit　a　high　surface　area　of　1136.40　m(2)/g,　together　with　a　pore　volume　of　0.39　cm(3)/g　and　an　average　pore　size　of　2.30　nm.　Used　as　stationary　phase　for　high-performance　liquid　chromatography　(HPLC),　the　particles　without　extra　bonding　either　C-18　or　C-8　can　successfully　separate　a　mixture　of　uracil,　phenol,　pyridine,　methylbenzene,　ethylbenzene　and　tert-butylbenzene.　The　obtained　materials　also　show　practical　application　in　the　separation　of　phthalate　acid　esters　(PAEs),　which　are　harmful　to　environment　and　human　health.　Although　the　columns　packed　with　ethyl-bridged　organic-inorganic　hybrid　silica　show　lower　column　efficiency　and　peak　symmetry　compared　to　commercial　column,　they　have　considerably　higher　chemical　stability　in　alkaline　mobile　phase.　The　HSS　column　also　possesses　high　mechanical　stability　which　is　similar　to　that　of　the　commercial　column.