The　mature　landfill　leachate　was　adopted　to　cultivated　anaerobic　ammonia　oxidation　bacteria　in　the　UASB　reactor　which　fed　with　inorganic　wastewater,　the　running　performance　of　the　system　was　explored　in　different　substrate　concentration　and　hydraulic　retention　time,　the　activity　inhibition　of　anaerobic　ammonia　oxidation　by　substrate　and　landfill　leachate　and　the　inhibition　kinetics　were　analyzed.　The　system　adapted　to　the　landfill　leachate　gradually　and　implement　efficient　denitrification　when　the　system　run　75d.　The　removal　amount　of　substrate　was　increased　at　first　and　then　decreased　with　the　increasement　of　the　substrate　concentration.　As　the　extension　of　HRT,　the　concentrations　of　substrate　and　landfill　leachate　was　increased,　the　effect　of　denitrification　was　decline　in　system.　The　thresholds　of　substrate　inhibition　were　that　NH4+-N　concentration　was　489.03　mg/L　and　NO2--N　concentration　of　192.36　mg/L.　when　NH4+-N　was　inhibitor,　Vmax　(NH4+-N)　was　0.1893　mg/(mg·d),　the　half-saturation　constant　was　39.39　mg/L,　the　inhibiting　kinetic　constant　was　3482.27　mg/L;　when　NO2--N　was　inhibitor,　Vmax　(NO2--N)　was　0.246　mg/(mg·d),　the　half-saturation　constant　was　43.19　mg/L,　the　inhibiting　kinetic　constant　was　701.15　mg/L.　Anaerobic　ammonia　oxidation　is　very　susceptible　especially　by　the　landfill　leachate,　the　activity　of　anammox　was　inhibited　completely　when　the　landfill　leachate　concentration　was　1450.69　mg/L　(which　calculated　in　COD).　©　2016,　Editorial　Board　of　China　Environmental　Science.　All　right　reserved.