Partial-denitrification　(PD,　NO3　-　N　-＞　NO2　-　N)　has　been　successfully　developed　to　produce　nitrite　(NO2)　with　acetate　as　the　sole　carbon　source.　In　this　study,　a　PD　sequencing　batch　reactor　(SBR)　fed　with　high-nitrate　(NO3　:　1000　mg　N/L)　was　firstly　developed　with　carbon　source　obtained　from　domestic　wastewater　(NH4+　:　59.3　mg　N/L,　chemical　oxygen　demand　(COD):　186.6　mg/L)　as　well　as　a　small　quantity　of　external　organic　matter.　The　results　showed　that　PD　was　not　degenerated　with　domestic　wastewater　addition,　and　a　high　nitrite　production　was　still　achieved　with　the　nitrate-to-nitrite　transformation　ratio　(NTR)　maintained　at　90%　in　the　173-days　operation　period.　By　optimizing　the　nitrate　and　domestic　wastewater　feeding　volume　as　well　as　external　carbon　dosage,　the　PD　effluent　was　suitable　for　ANAMMOX　process　with　a　NO2　-　N/NH4+-N　of　1.46　and　COD/NO2　-　N　of　1.32　obtained　under　the　influent　volume　of　domestic　wastewater　of　2.8　L,　as　well　as　nitrate　of　0.4　L,　and　the　external　carbon　demand　was　reduced　to　COD/NO3　-　N　of　1.7.　Nitrate　reduction　rate　was　fit　to　Monod　kinetics,　with　a　maximum　specific　NO3　-　N　reduction　rate　of　4.56　g　N/g　VSS/d　and　nitrate　half-saturation　constant　of　6.9　mg　N/L,　respectively.　High-throughput　sequencing　analysis　revealed　that　the　diversity　of　microbial　communities　was　increased　with　the　domestic　wastewater　added,　but　the　denitrifiers　of　Thaura　genus　that　was　responsible　for　high　nitrite　production　was　still　dominant　in　PD　reactor　with　a　percentage　of　54.9-72.9%.　This　study　indicated　that　the　high-nitrate　and　domestic　wastewater　could　be　simultaneously　treated　via　the　novel　PD-ANAMMOX　process　in　a　cost-effective　way.　(C)　2017　Elsevier　B.V.　All　rights　reserved.