An　air-cathode　microbial　fuel　cell　(ACMFC)　was　constructed　by　using　Pt/C　as　cathode,　graphite　felt　as　anode　and　glucose　as　the　anode　fed.　The　effects　of　fluid　conditions　and　concentrations　of　glucose　on　electrochemical　properties　were　studied.　Results　show　that　the　maximum　power　of　approximately　85.9　mW/m2　is　obtained　under　the　fluid＇s　velocity　15　mL/min　and　density　of　glucose　1.0　g/L.　The　COD　removal　efficiency　of　ACMFC　is　81.6%　after　running　48　h.