Many　regions　in　Canada　are　facing　increasing　environmental　threats　posed　by　oil　and　gas　exploitation　and　transportation.　These　contaminated　lands　are　inevitably　subjected　to　seasonal　and　diurnal　freeze-thawing　cycles　(FTCs).　However,　knowledge　about　the　effect　of　FTCs　on　the　behaviours　of　hydrophobic　contaminants　during　the　aging　process　of　soil　is　limited.　This　study　investigated　the　desorption　characteristics　of　phenanthrene　in　aging　soils　in　the　presence　of　the　biosurfactant　rhamnolipid　under　diurnal　and　seasonal　FTC　treatments.　It　was　found　that　the　presence　of　rhamnolipid　in　soil　during　the　aging　process　was　able　to　increase　the　desorption　efficiency　of　phenanthrene.　In　the　presence　of　rhamnolipid　above　100　mg　L-1,　FTCs　could　inhibit　the　sequestration　of　phenanthrene.　Soil　moisture　and　rhamnolipid　concentration　are　two　major　factors　affecting　this　effect.　High　moisture　content　and　FTC　frequency　could　lead　to　lower　desorption　in　the　early　stage　of　FTCs　due　to　the　increased　specific　surface　area.　The　sequestration　of　phenanthrene　was　less　effectively　hindered　under　seasonal　FTCs　than　diurnal　FTCs.　The　results　from　this　study　have　important　implications　for　understanding　the　role　of　surfactants　in　cold-region　soil　aging,　and　for　the　improvement　of　site　remediation　strategies　of　PAH　contaminated　soil　in　cold　regions.