Micro　total　analysis　system　or　Lab-on-a-chip,　in　which　microfluidic　chips　are　significant　components,　has　caused　wide　attention　in　recent　years.　CD/DVD　manufacturing　technology　is　a　highly　efficient　and　low-cost　solution　to　fabricate　microfluidic　chips.　In　this　paper,　microfluidic　chips　made　of　polycarbonate　(PC)　were　firstly　fabricated　by　CD/DVD　manufacturing　technology.　The　manufacturing　process　is　divided　to　five　main　sub-processes　which　are　photomask　fabrication,　photolithography,　electroforming,　stamping　and　thermal　bonding.　Besides,　by　repeatedly　bonding　for　three　times,　the　bonding　success　percentage　of　the　chip　could　reach　about　98　%.　The　surface　roughness　of　the　microstructure　in　microfluidic　chips　which　has　significant　influences　on　thermal　bonding　success　percentage　and　test　results　are　also　detailedly　studied.　Surface　roughness　of　SU-8　pattern,　Ni　stamper　and　cover　slips　of　PC　microfluidic　chips　was　measured　and　analyzed.　In　the　process　of　electroforming　and　stamping,　the　surface　roughness　is　increased.　Moreover,　influences　of　cover　slips＇　surface　roughness　on　thermal　bonding　are　analyzed　by　bonding　five　groups　of　cover　slips　on　PC　plates　with　different　average　surface　roughness.　The　thermal　bonding　success　percentage　is　obviously　reduced　with　the　increase　of　average　surface　roughness.