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学者姓名：杨宏
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Abstract ：
By embedding immobilized biofiller, 100% denitrification efficiency was achieved with nitrate or nitrite as electron acceptors utilizing volatile fatty acids (VFAs) from domestic wastewater after hydrolysis acidification. The consumption patterns of VFAs by functional bacteria and differences in nitrogen metabolic gene expression were thoroughly analyzed. Total consumption of acetic and propionic acids with >95% VFAs utilization was achieved utilizing nitrate, whereas the consumption of butyric and valeric acids was enhanced utilizing nitrite. Denitrification-related genes were all upregulated, particularly nosZ, indicating systemic N2O emission reduction potential. Electron acceptor changes dynamically shifted microbial dominance from Thauera (19.4%) to Thiobacillus (7.2%). These results provide valuable insights into the adaptability and ecological niche characterization of denitrifying bacteria, contributing to improving nitrogen removal efficiency, optimizing carbon source utilization, and reducing sludge production.
Keyword ：
Microbial community dynamics Microbial community dynamics Nitrogen removal Nitrogen removal Organic carbon utilization Organic carbon utilization Immobilized technology Immobilized technology Denitrification electron acceptors Denitrification electron acceptors
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| GB/T 7714 | Li, Siqi , Yang, Hong . Optimizing denitrification with volatile fatty acids from hydrolysis acidification-treated domestic wastewater: Comparative effects of nitrate and nitrite using immobilized biofiller [J]. | JOURNAL OF ENVIRONMENTAL MANAGEMENT , 2025 , 378 . |
| MLA | Li, Siqi 等. "Optimizing denitrification with volatile fatty acids from hydrolysis acidification-treated domestic wastewater: Comparative effects of nitrate and nitrite using immobilized biofiller" . | JOURNAL OF ENVIRONMENTAL MANAGEMENT 378 (2025) . |
| APA | Li, Siqi , Yang, Hong . Optimizing denitrification with volatile fatty acids from hydrolysis acidification-treated domestic wastewater: Comparative effects of nitrate and nitrite using immobilized biofiller . | JOURNAL OF ENVIRONMENTAL MANAGEMENT , 2025 , 378 . |
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Abstract ：
The current sewage treatment systems are typically unable to efficiently remove both nitrogen and phosphorus simultaneously, often compromising biological phosphorus removal to ensure effective nitrogen removal. To resolve this issue, this study establishes an independent enhanced biological phosphorus removal (EBPR) system based on an immobilized fillers nitrogen removal system for treating actual rural sewage. The research focuses on the phosphorus removal performance, operational conditions, and characteristics of the independent EBPR system. When fluctuations occur in the effluent phosphorus concentration, phosphorus-accumulating organisms (PAOs) utilize only 29 % of the available carbon sources, with the remaining carbon being fully consumed by glycogen-accumulating organisms (GAOs). The presence of GAOs and low dissolved oxygen (DO) concentrations leads to deteriorating phosphorus removal performance in the system. To address this, reducing the carbon load and increasing aeration are necessary to maintain effluent phosphorus concentrations below 0.5 mg & sdot;L- 1. Tetrasphaera and Acinetobacter are identified as key microbial genera responsible for phosphate removal in this system. Finally, fluorescence excitation emission matrix parallel factor analysis (EEM-PARAFAC) showed that fermentation microorganisms, represented by Tetrasphaera, played a key role in the removal of tryptophan and tyrosine produced by raw wastewater and microbial metabolism.
Keyword ：
Immobilized fillers Immobilized fillers Microbial community structure Microbial community structure Parallel factor analysis Parallel factor analysis Independent enhanced biological phosphorus removal Independent enhanced biological phosphorus removal
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| GB/T 7714 | Chen, Wenhao , Yang, Hong , Wang, Xiaotong et al. Experimental study on the establishment of independent enhanced biological phosphorus removal system based on immobilized fillers for wastewater nitrogen removal [J]. | JOURNAL OF WATER PROCESS ENGINEERING , 2025 , 70 . |
| MLA | Chen, Wenhao et al. "Experimental study on the establishment of independent enhanced biological phosphorus removal system based on immobilized fillers for wastewater nitrogen removal" . | JOURNAL OF WATER PROCESS ENGINEERING 70 (2025) . |
| APA | Chen, Wenhao , Yang, Hong , Wang, Xiaotong , Liu, Weichao . Experimental study on the establishment of independent enhanced biological phosphorus removal system based on immobilized fillers for wastewater nitrogen removal . | JOURNAL OF WATER PROCESS ENGINEERING , 2025 , 70 . |
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Abstract ：
A gel filler with diatomaceous earth, activated carbon, and CaCO3 compounded with polyvinyl alcohol was prepared and optimized. Rapid start-up and efficient and stable operation of partial nitrification (PN) was achieved by immobilization of conventional activated sludge. The results showed that the proper material proportion synergistically improved the performance and oxygen mass transfer rate of the gel. The continuous flow reactor was operated for 10d to successfully start PN, and the maximum ammonia oxidation rate of this filler was 58.30 mg center dot(L center dot h)- 1 during the stable operation, and the nitrite accumulation rate was maintained above 90 %. However, due to the greater mass transfer resistance of unoptimized fillers, more dissolved oxygen waste and nitrogen loss were caused, resulting in delayed expression of biological activity. Based on the kinetic characterization of ammonia-oxidizing bacteria (AOB) within the filler and high-throughput sequencing, it was demonstrated that the rapid start-up and efficient and stable operation of the PN system was due to the highly oxygenophilic and community-dominant position of AOB within this filler. This study provides new options and insights at the level of immobilization technology for the realization and efficient and stable operation of PN.
Keyword ：
Immobilization materials Immobilization materials Partial nitrification Partial nitrification Nitrogen removal Nitrogen removal Start-up Start-up Oxygen mass transfer Oxygen mass transfer
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| GB/T 7714 | Zhang, Xinming , Yang, Hong . Optimize the ratio of immobilized gel materials to improve gel performance and oxygen mass transfer rate: Achieve rapid start-up and efficient and stable operation of partial nitration [J]. | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING , 2024 , 12 (5) . |
| MLA | Zhang, Xinming et al. "Optimize the ratio of immobilized gel materials to improve gel performance and oxygen mass transfer rate: Achieve rapid start-up and efficient and stable operation of partial nitration" . | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING 12 . 5 (2024) . |
| APA | Zhang, Xinming , Yang, Hong . Optimize the ratio of immobilized gel materials to improve gel performance and oxygen mass transfer rate: Achieve rapid start-up and efficient and stable operation of partial nitration . | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING , 2024 , 12 (5) . |
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Abstract ：
A two-stage partial nitritation/anammox process based on microbial encapsulation (PN/A-E) was established. The nitrogen removal characteristics of PN/A-E under high and low ammonia nitrogen and after reactivation following a long-term shutdown were comprehensively investigated and compared with anammox granular sludge (AnGS). The stable PN process did not depend on high ammonia nitrogen, and the nitrite accumulation rate reached 95.2 +/- 0.7 %. The overall nitrogen removal rate of encapsulated anammox bacteria was twice that of the AnGS, and it was more tolerant to external interference. Moreover, PN/A-E showed good reactivation performance, and the total nitrogen in the effluent was 10.0 +/- 1.4 mg center dot L-1 when the final hydraulic retention time was 2.18 h. The immobilized fillers support an increase in ammonia-oxidizing bacteria under restricted conditions and were more conducive to the dominance of functional bacteria and the stability of microbial community under low ammonia nitrogen. This study provides a positive method to achieve a reliable PN/A.
Keyword ：
Microbial community structure Microbial community structure Anammox Anammox Nitrogen removal Nitrogen removal Partial nitritation Partial nitritation Microbial immobilization Microbial immobilization
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| GB/T 7714 | Wang, XiaoTong , Yang, Hong , Wang, JiaWei . Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes [J]. | BIORESOURCE TECHNOLOGY , 2023 , 370 . |
| MLA | Wang, XiaoTong et al. "Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes" . | BIORESOURCE TECHNOLOGY 370 (2023) . |
| APA | Wang, XiaoTong , Yang, Hong , Wang, JiaWei . Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes . | BIORESOURCE TECHNOLOGY , 2023 , 370 . |
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Abstract ：
The present study sought to treat the ion-adsorption type rare earth mine wastewater in a pilot plant system using the nitritation-denitrification process with immobilized biological fillers. Meanwhile, the microbial community structure in the immobilized fillers was analyzed. The ammonia nitrogen removal rate of the nitritation fillers reached 0.43 kg N m−3 d−1 on day 61 (28.1 °C) and the total nitrogen removal rate of the denitrification fillers reached 0.51 kg N m−3 d−1 on day 58 (27.8 °C) under the hydraulic retention time of 3.4 hr and a fillers volume ratio of 8% (v/v). The average NH4+-N and TN removal efficiencies of the nitritation-denitrification process were 96.9% and 96.8%, respectively, during the steady state operation period (day 26–72). The results also indicate that high DO (5.5–6.5 mg L−1) could induce rapid growth of nitrite-oxidizing bacteria (NOB) in the nitritation fillers, even though the initial NOB proportion is extremely low. The optimal operational performance of the denitrification fillers reactor was the COD/(NO2−-N + NO3−-N) ratio of 3.7–4.2. The high-throughput sequencing analysis showed that the functional bacteria in the immobilized fillers could maintain a competitive advantage. The abundance of Nitrosomonas in the nitritation fillers increased from 6.58% to 21.24%, while Thauera and Ottowia were the dominant denitrifying bacteria genera in the denitrification fillers. © 2022 Elsevier B.V.
Keyword ：
Denitrification; Immobilized biological fillers; Microbial community structure; Nitritation; Rare earth mine wastewater Denitrification; Immobilized biological fillers; Microbial community structure; Nitritation; Rare earth mine wastewater
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| GB/T 7714 | Zou, Z. , Yang, H. , Zhang, S. et al. Nitrogen removal performance and microbial community analysis of immobilized biological fillers in rare earth mine wastewater [J]. | Biochemical Engineering Journal , 2022 , 186 . |
| MLA | Zou, Z. et al. "Nitrogen removal performance and microbial community analysis of immobilized biological fillers in rare earth mine wastewater" . | Biochemical Engineering Journal 186 (2022) . |
| APA | Zou, Z. , Yang, H. , Zhang, S. , Chi, W. , Wang, X. , Liu, Z. . Nitrogen removal performance and microbial community analysis of immobilized biological fillers in rare earth mine wastewater . | Biochemical Engineering Journal , 2022 , 186 . |
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Abstract ：
The nitrogen removal characteristics, physicochemical properties, and microbial community composition of four different anaerobic ammonium oxidation (anammox) sludge morphologies were investigated. The morphologies considered in this study, namely suspended sludge (Rs), biofilm (Rm), granular sludge (Rg), and encapsulated biomass (Re), were prepared from floc sludge. The results show that Re exhibited the maximum anammox activity, followed by Rg, Rm, and Rs. Additionally, the anammox contribution rate was higher in Rg and Re. The higher extracellular polymer content in Rg promoted sludge accumulation, and tryptophan was observed in Rm and Rg, which was replaced by humic acids in Rs. Re showed the largest specific surface area, hydrophobicity and strength, and its good structure ensured enrichment of anammox bacteria (AnAOB). In terms of the microbial community, the functional bacterium Candidatus Kuenenia accounted for the highest proportion in Rm (39.27%), but the presence of both anaerobic and aerobic regions led to increased community complexity with more nitrifying bacteria. In contrast, Rg and Re had a more specific microbial community. In addition, denitrifying bacteria tended to grow in Rs, while nitrifying bacteria were retained in Rm. The AnAOB were more likely to be enriched in sludge aggregates (both Rm and Rg) and carriers (Re). Through correlation analysis, the potential relationship involving bacterial flora evolution of each sample was clarified. Finally, the structural models of different morphologies of sludge were proposed. This study deepens the understanding of various anammox sludge morphologies as well as provides useful information for the cultivation of AnAOB and further application of anammox.
Keyword ：
Anammox Anammox Granular sludge Granular sludge Flora structure Flora structure Immobilized filler Immobilized filler Biofilm Biofilm Sludge morphology Sludge morphology
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| GB/T 7714 | Wang, XiaoTong , Yang, Hong , Su, Yang et al. Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community [J]. | CHEMOSPHERE , 2022 , 288 . |
| MLA | Wang, XiaoTong et al. "Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community" . | CHEMOSPHERE 288 (2022) . |
| APA | Wang, XiaoTong , Yang, Hong , Su, Yang , Liu, XuYan . Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community . | CHEMOSPHERE , 2022 , 288 . |
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Abstract ：
Four anaerobic ammonium oxidation (anammox) immobilized filler reactors (R1: 33 degrees C-normal, R2: seasonal temperature-normal, R3: seasonal temperature-feast, R4: seasonal temperature-starvation) were established to study the response of anammox immobilized fillers to seasonal temperature changes and different operating modes. The results showed that the anammox immobilized filler could better adapt to the seasonal temperature drop and maintain the activity potential by adjusting the hydraulic retention time (HRT). During the temperature rise phase, R2 activity increased rapidly with the highest nitrogen removal rate reaching 1.26 kgN center dot(m3 center dot d)-1, which was equivalent to control sample R1 (1.33 kgN center dot(m3 center dot d)-1). However, feasting and famine conditions severely impaired anammox performance and changed stoichiometric ratios; feasting, in particular, significantly lowered the nitrogen removal potential of R3. The specific anammox activity of R2, R3 and R4 was 92.2%, 52.6% and 67.9%, respectively, that of R1, respectively, where the accumulation of functional bacteria was the reason for the higher activity of R2. Degradation kinetics and NO2--N inhibition curves showed that R3 was less sensitive to high concentrations of NH4+-N, while R4 responded earlier to low concentrations of NH4+-N, and the reduction of IC50 at low temperature was the reason for the inhibition of R3 activity. Furthermore, seasonal temperature fluctuations had little effect on the microbial community structure but had a considerable impact on bacteria abundance. The anammox functional bacteria Candidatus Kuenenia was found to be the dominant genus in R1-R4; however, the relative abundance of most bacteria, including anammox bacteria, decreased in R3, while the proportion of fermentation bacteria and denitrifying bacteria increased in R4. These findings highlight the necessity of rational regulation of HRT for the adaptation of anammox immobilized fillers to seasonal temperature changes, which could enhance our understanding of the synergistic effect of seasonal temperature changes and different operating modes on nitrogen removal.
Keyword ：
Anammox Anammox Immobilized fillers Immobilized fillers Microbial community Microbial community Feast-famine condition Feast-famine condition Seasonal temperature change Seasonal temperature change
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| GB/T 7714 | Wang, XiaoTong , Yang, Hong . Nitrogen removal performance of anammox immobilized fillers in response to seasonal temperature variations and different operating modes: Substrate utilization and microbial community analysis [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 829 . |
| MLA | Wang, XiaoTong et al. "Nitrogen removal performance of anammox immobilized fillers in response to seasonal temperature variations and different operating modes: Substrate utilization and microbial community analysis" . | SCIENCE OF THE TOTAL ENVIRONMENT 829 (2022) . |
| APA | Wang, XiaoTong , Yang, Hong . Nitrogen removal performance of anammox immobilized fillers in response to seasonal temperature variations and different operating modes: Substrate utilization and microbial community analysis . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 829 . |
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Abstract ：
In this study, the effects of different storage conditions, such as temperature, storage time and biomass form, on the properties of anaerobic ammonia oxidation (anammox) were investigated along with the identification of the process mechanism. The results showed that the influence of storage time on anammox properties was stronger than that of storage temperature and biomass form. Also, the anammox recovery activity at 15 degrees C was better than that at 4 degrees C, and the anammox recovery activity of immobilized filler was better than that of anammox granular sludge (AnGS). Although cryogenic storage severely damaged anammox activity, lower loss of extracellular polymeric substances maintained the AnGS structure. The maximum recovery of specific anammox activity at 15 degrees C for the immobilized filler was observed to be 109%. In addition, intermittent substrate supplementation weakened the adverse effect of long-term storage on anammox activity, and was conducive to maintaining stable flora composition and promoting regeneration of anammox bacteria (AnAOB). High-throughput sequencing analysis showed that starvation resulted in increased community diversity, and the functional bacteria Candidatus Brocadia was observed to be more tolerant to starvation than Candidatus Kuenenia. Finally, principal component analysis was used to explain the complex relationship between process performance and preservation conditions. Based on the results of this work, it is recommended to preserve AnAOB in the form of immobilized filler at 15 degrees C and supplement substrate intermittently during long term storage. This study provides an economical and robust strategy for the short-term and long-term preservation of AnAOB. (c) 2021 Elsevier B.V. All rights reserved.
Keyword ：
Preservation conditions Preservation conditions Active recovery Active recovery Immobilized filler Immobilized filler Flora structure Flora structure Anammox bacteria Anammox bacteria
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| GB/T 7714 | Wang, XiaoTong , Yang, Hong , Geng, LiangHan et al. Analyzing the effect of storage conditions on anammox recovery performance from the perspectives of time, temperature and biomass form [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 816 . |
| MLA | Wang, XiaoTong et al. "Analyzing the effect of storage conditions on anammox recovery performance from the perspectives of time, temperature and biomass form" . | SCIENCE OF THE TOTAL ENVIRONMENT 816 (2022) . |
| APA | Wang, XiaoTong , Yang, Hong , Geng, LiangHan , Liu, XuYan . Analyzing the effect of storage conditions on anammox recovery performance from the perspectives of time, temperature and biomass form . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 816 . |
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Abstract ：
The immobilized carrier was prepared with complete anaerobic ammonia oxidation granular sludge (AnGS) and crushed AnGS, respectively. We evaluated the effects of granular form and continuous temperature changes on nitrogen removal by immobilized anaerobic ammonium oxidation (anammox) filler. The results showed that the rate of nitrogen removal of crushed and encapsulated AnGS was 20% higher than that of direct encapsulated AnGS. However, the latter had higher thresholds of tolerance to Fe2+ and Cu2+. In addition, the immobilization reduced the activation energy of anammox. Above 12.5 degrees C, the immobilized filler was efficient at removing nitrogen removal through the dual adjustment of temperature-hydraulic retention time. From 12.5 degrees C to 23 degrees C, the temperature had a greater influence on the nitrogen removal effect than the HRT. In contrast, HRT had a dominant influence from 23 degrees C to 32 degrees C. Anammox activity was severely inhibited below 12.5 degrees C. High-throughput sequencing analysis showed that the community structure migrated with the changes in temperature. The anammox functional bacteria Candidatus Kuenenia (18.31-39.73%) were the dominant genus at medium and high temperatures, and it was replaced by Chryseobacterium (24.19%) at 8.5 degrees C. In addition, an RDA analysis showed that Candidatus Brocadia was more adaptable to low temperatures than Candidatus Kuenenia. In addition, Bellilinea was more sensitive to temperature than Candidatus Kuenenia. Thus, the temperature could be appropriately lowered to avoid overbreeding. The results of this study optimized the operation of an anammox immobilized system and promote its further application. (C) 2021 Elsevier B.V. All rights reserved.
Keyword ：
Anammox Anammox Temperature Temperature AnGS form AnGS form Metal ions Metal ions Microflora structure Microflora structure Immobilized filler Immobilized filler
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| GB/T 7714 | Wang, XiaoTong , Yang, Hong , Su, Yang et al. Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 . |
| MLA | Wang, XiaoTong et al. "Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis" . | SCIENCE OF THE TOTAL ENVIRONMENT 803 (2022) . |
| APA | Wang, XiaoTong , Yang, Hong , Su, Yang , Liu, XuYan . Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 . |
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Abstract ：
为优化短程硝化反硝化工艺对模拟城市污水的脱氮处理性能，通过包埋固定化技术分别制得短程硝化、反硝化填料，形成连续流脱氮工艺。结果表明，短程硝化填料活性恢复后的氨氧化速率(AOR)可达39.83mg·(L·h)-1，亚硝酸盐积累率(NAR)稳定在96.60%。采用响应曲面法考察了短程硝化过程中的DO、HRT和填充率等因素对氨氮去除率(ARR)和NAR的影响，并建立了二次回归模型，通过模型预测的最佳运行工况为：HRT为3.48 h,DO为3.64 mg·L~(-1)，填充率为20%。此时，后置反硝化包埋填料，当平均C/N为2.82时，总氮出水平均质量浓度为2.29 mg·L~(-1)，去除率稳定在9...
Keyword ：
反硝化 反硝化 短程硝化 短程硝化 包埋填料 包埋填料 响应曲面法 响应曲面法
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| GB/T 7714 | 方小月 , 杨宏 , 刘旭妍 et al. 基于包埋填料的短程硝化反硝化工艺的脱氮性能优化 [J]. | 环境工程学报 , 2022 , 16 (06) : 1979-1990 . |
| MLA | 方小月 et al. "基于包埋填料的短程硝化反硝化工艺的脱氮性能优化" . | 环境工程学报 16 . 06 (2022) : 1979-1990 . |
| APA | 方小月 , 杨宏 , 刘旭妍 , 王晓曈 , 胡鑫 . 基于包埋填料的短程硝化反硝化工艺的脱氮性能优化 . | 环境工程学报 , 2022 , 16 (06) , 1979-1990 . |
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